Protein crystallization using surface engineering: towards a general recipe
نویسندگان
چکیده
منابع مشابه
Entropy and surface engineering in protein crystallization.
Protein crystallization remains a key limiting step in the characterization of the atomic structures of proteins and their complexes by X-ray diffraction methods. Current data indicate that standard screening procedures applied to soluble well folded prokaryotic proteins yield X-ray diffraction crystals with an approximately 20% success rate and for eukaryotic proteins this figure may be signif...
متن کاملProtein surface shielding agents in protein crystallization
The molecules adhering temporarily on the surface of protein molecules change the propensity of protein molecules to deposit on the crystal surface in a definite position and orientation. The concepts of competitive adhesion modes and protein surface shielding agents acting on the surface of molecules in a non-equilibrium process of protein crystallization provide a useful platform for the cont...
متن کاملTowards Crystallization using Optical Tweezers
Recently we have shown that protein crystals could be grown while they were three-dimensionally trapped by optical tweezers. This permitted studies of modifications of single crystals while gradually changing the conditions in the growing solution. Furthermore it allowed the crystals to grow far away from container walls favoring high quality crystal growth. Many protein crystals themselves con...
متن کاملNIRCam Systems Engineering: The Recipe
NIRCam is a highly sensitive, multi-spectral, high-resolution, cryogenic, compact, light-weight, and rugged near infrared camera on the James Webb Space Telescope. The successful development of NIRCam is aided by good system engineering practice. This is a discussion of important elements of NIRCam System Engineering with some lighthearted contrast to a more common activity.
متن کاملCombined protein construct and synthetic gene engineering for heterologous protein expression and crystallization using Gene Composer
BACKGROUND With the goal of improving yield and success rates of heterologous protein production for structural studies we have developed the database and algorithm software package Gene Composer. This freely available electronic tool facilitates the information-rich design of protein constructs and their engineered synthetic gene sequences, as detailed in the accompanying manuscript. RESULTS...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The FASEB Journal
سال: 2006
ISSN: 0892-6638,1530-6860
DOI: 10.1096/fasebj.20.4.a526-d